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Understanding DNA damage is central to modern biology. Whether researchers are studying cancer, neurodegenerative disease, genome stability, DNA replication or therapeutic safety, one question continues to emerge.

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, Gene Editing,

This technical white paper describes the ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® Analysis Software and explains the advantages of adopting a purpose-built, verified solution rather than developing and maintaining an equivalent pipeline in-house. Engineered in accordance with industry-standard development practices, the software is highly configurable, delivers consistent high­quality outputs, and is supported by a formal Software Verification Plan and Report filed within Broken String Bioscience's FDA Master Access File (MAF).

Whitepaper

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, Gene Editing, Bioinformatics

This technical note explains the analytical logic, describes the principal outputs, and explains how each should be read. For guidance on experimental design and timepoint selection, refer to the companion technical note, Designing ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® Experiments for Nuclease-Based Gene Editing.

Technical Note

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, Off-target, On-target, Gene Editing

Explore how ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® enables unbiased, genome-wide detection of on- and off-target DNA breaks in therapeutically relevant cells across CRISPR nucleases and base editors. This poster highlights applications in guide screening, off-target characterization, and patient-derived iPSC workflows, supporting safer gene editing development from discovery through IND-enabling studies

Poster

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, ABE, CBE

An overview of the ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® platform, detailing its PCR-free, cell-based approach to genome-wide DNA break mapping. This technical note outlines the scientific basis, workflow, and outputs, and explores how INDUCE-seq enables accurate, unbiased characterization of on- and off-target activity across gene editing programs.

Technical Note

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, Off-target, On-target, Gene Editing

In this recent webinar brought to you by The Scientist, Our COO and co-founder Felix Dobbs presents how new developments are supporting the translation of gene editing technologies into cell and gene therapy applications. 

Webinar

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, Gene Editing

This technical note provides structured guidance on the key experimental design decisions required to generate robust, interpretable ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® data. It covers the factors that affect break kinetics, the rationale for different control types, approaches to timepoint selection across editing systems and delivery methods, and considerations for balancing experimental depth with practical constraints.

Technical Note

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, Nucleases, Gene Editing

In a new featured article, our COO & Co-founder, Felix Dobbs, breaks down the effects of CRISPR-Cas off-target activity, where off-target screening is falling behind and what the future of off-target screening looks like.

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

In April 2026, the FDA released critical new draft guidance for genome editing safety. In the document the FDA established what they expect for NGS-based off-target assessment in IND-enabling studies for gene therapy products. This flyer shows how ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® meets every part of the FDA guidance criteria.

Flyer

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

Genome-wide off-target mapping technologies have advanced rapidly in recent years. It’s now routine to generate hundreds to thousands of putative off-target sites from a single experiment. Detection sensitivity has improved. Sequencing costs have fallen. Throughput has increased. Yet the critical question remains surprisingly difficult to answer: Which of these sites matter? 

Blog

Gene Editing, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, Bioinformatics

This study screens 120 dual-gRNA pairs in patient iPSCs to identify efficient and safe CRISPR/Cas9 candidates for excising the C9orf72 repeat expansion — the leading genetic cause of frontotemporal dementia and ALS. Off-target analysis combining whole-genome sequencing and ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® revealed only one confirmed off-target across the four lead gRNAs, with ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® uniquely detecting it without prior nomination or false positives, establishing a robust framework for dual-gRNA screening applicable to other repeat expansion disorders.

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, CRISPR, Gene Editing

To mitigate the risk of off-target editing, the FDA now requires unbiased, empirical measurement of off-target activity to be produced for every gene therapy candidate

Poster

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High-Throughput, Gene Editing, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

Genome editing holds enormous therapeutic promise; however, unintended DNA damage remains a critical barrier. Off-target effects are difficult to predict, hard to detect, and continue to carry serious safety risks.

Flyer

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Gene Editing, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

Video

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Assays, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

Accurately measuring DNA double strand breaks (DSBs) is critical for assessing DNA damage and developing safe genome editing therapies, but current methods are limited by noise, poor sensitivity, and high costs. INDUCE-seq® overcomes these challenges by simultaneously detecting both low-level endogenous and induced DSBs via a novel NGS flow cell enrichment approach, enabling characterisation of repair mechanisms and safer therapeutic development.

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DSBs, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, DNA

Video

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Assays, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

±õ±·¶Ù±«°ä·¡-²õ±ð±ç® is a genome-wide, in cellulo platform for the direct detection and quantification of DNA double-strand breaks. Rather than extracting genomic DNA first and labelling break ends later, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® performs in situ break labelling within fixed and permeabilised cells. This preserves the genomic context of break events as they existed inside the cell. It avoids the distortions introduced by post-extraction manipulation and PCR amplification. 

Blog

Gene Editing, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, DNA

This user guide provides detailed instructions for performing the ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® Analysis workflow, including starting an analysis run, navigating the BSB ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® Analysis Workspace, and understanding the generated output reports. For information on preparing next-generation sequencing (NGS) libraries using the On-Demand ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® Solution Assay, refer to the on-demand ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® Solution Assay user guide.

User Guide

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Assays, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

This guide explains how to prepare next-generation sequencing (NGS) libraries using the INDUCE-seq® assay. The INDUCE-seq® assay workflow is specifically designed to measure an characterize genome-wide DNA breaks from cell samples. This workflow is based on the INDUCE-seq® technology to enable sensitive, unbiased detection of gene-editing off-target events, providing critical insights into the safety and efficacy of gene-editing therapies.

User Guide

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Assays, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

Protocol

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Assays, ±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

When people talk about gene editing safety, the focus is usually on detection: where are the off-targets? How many are there? Can we trust the data? However, many issues start much earlier within the experimental design. If the design isn’t right, the data won’t be either.

Blog

±õ±·¶Ù±«°ä·¡-²õ±ð±ç®

A multi-institutional research collaboration, led by Imperial College London, used ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® to map genome-wide DNA double-strand breaks and uncover how disruption of the miR-346:NORAD pathway contributes to genome instability in prostate cancer. The findings provide new insights into disease mechanisms and highlight a potential therapeutic target.

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, DNA

Researchers at Imperial College London combined ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® with biophysical approaches to investigate why some predicted CRISPR-Cas9 off-target sites are cleaved in cells while others are not. The study demonstrated how DNA structure influences off-target editing, providing new insights into genome editing specificity.

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, DNA, Off-Target

Researchers at the MRC Laboratory of Molecular Biology used ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® to investigate how DNA replication contributes to DNA break formation. Their findings showed that replication origin activation itself can generate DNA double-strand breaks and mutations, providing new insights into the mechanisms that shape genome stability.

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, DNA

A multi-institutional research collaboration led by researchers at the University of Birmingham used ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® to investigate the role of USP50 during DNA replication. The study showed that loss of USP50 increased DNA breaks, helping to reveal how cells protect stalled replication forks and maintain genome stability.

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, DNA

A multi-institutional research collaboration led by researchers at the Institute of Cancer Research used ±õ±·¶Ù±«°ä·¡-²õ±ð±ç® to investigate how cells protect G-quadruplex DNA structures from damage. The study showed that loss of SMARCA4 increases DNA breaks at these sites, providing new insights into genome stability and identifying potential therapeutic vulnerabilities in cancer. 

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±õ±·¶Ù±«°ä·¡-²õ±ð±ç®, DNA